What is the method of using HIV saliva test paper? Teach you how to use HIV saliva test paper correctly

We all know that because of the openness of sex, AIDS is now rampant in the population. Now people may be suffering from AIDS, but they are not particularly sure, but they are embarrassed to go to the hospital for examination. At this time, people will choose to buy dwarf saliva test paper at home to test whether they have AIDS. But many people do not know how to use this test strip, so now I will teach everyone how to use it.

Recombinant antigens HIV-1gp41 and HIV-2gp36 were encapsulated in the detection area of the nitrocellulose membrane, and sheep anti human IgGF (ab ') 2 fragment polyclonal antibody was encapsulated in the control area. During testing, wipe the upper and lower gingival lines of the subject with an oral swab, and then place the oral swab in a container containing approximately 1000 μ Mix the sample buffer in the test tube evenly, squeeze the liquid from the oral swab dry, and discard the swab. About 200 μ Transfer the sample mixture of l to a clean test tube for testing.

When the test strip is vertically inserted into the test tube containing the sample mixture, the light red protein A-gold marker on the test strip will dissolve, and the IgG in the sample (other antibodies in the sample, such as IgA and IgM, have the same reaction principle as IgG) will combine with the protein A/gold particles to form the "IgG protein A-gold" complex, and the "IgG protein A-gold" complex will move upward along the test strip to reach the detection area coated with HIV antigen first.

If the sample contains HIV antibodies, the "IgG protein A-gold" complex will bind to the antigen and remain on the detection line, forming a light red line, indicating a positive result. The depth of the line color is not proportional to the number of antibodies in the sample. If there are no colored lines in the testing area, it indicates that the sample does not contain HIV antibodies. The free "IgG protein A gold" complex continues to move above the test paper and reaches the control area of the test paper. The control area was coated with 2 fragments of sheep anti human IgGF (ab ') and fixed on the control line of the test strip. The "IgG protein A gold" complex binds to the sheep anti human IgGF (ab ') 2 fragment and enriches on the control line, forming a light red line.

The appearance of the control line proves that the test strip detection function is normal. Regardless of whether the sample contains HIV-1/2 antibodies, a light red to purple control line should appear in the control area of the test strip in the effectiveness test. The sample continues to move and enters the absorption zone through the control area. The function of the absorption zone is to adsorb the remaining "IgG protein A gold" complex, causing it to move within the test strip and eliminate the background color.